Hek293 and HeLa cells are essential in research due to their adaptability and efficiency in transfection. Key steps in maintaining these cells include ensuring a sterile lab environment, selecting the appropriate media, and subculturing at the right density. Regular monitoring helps maintain cell health, while advanced techniques such as CRISPR/Cas9 and 3D culture methods allow for more sophisticated experiments, enabling progress in fields like gene editing, drug development, and cancer studies.
